Bio-techne – Jess
ProteinSimple’s Jess™ is a Simple Western™ instrument, an innovative technology from Bio-Techne, that performs capillary-based immunoassays and fully automated Western blot analysis, taking the complexity out of traditional immunoassays like Western blot and ELISA and filling in their shortcomings.
Technical Specifications
| Total Protein Specification | Chemiluminescence and Stellar Fluorescence Specification | Classic Fluorescence Specification | Protein Normalization Specification | |
| Sample required | 0.3-1.2 µg | 0.6-1.2 µg | 2-4 µg | 0.6-4 µg |
| Volume required | 3 µL/well | |||
| Size range | Molecular weight (MW) ladder ranges from 2-440 kDa | |||
| Sizing CV | <10% | |||
| Intra-assay CV | <15% | <20% | ||
| Inter-assay CV | <20%** | <20%* | <20% | <20% |
| Resolution (± percent difference in MW) | ± 15-20% for MW <20 kDA ± 10% for MW >20 kDa | |||
| Quantitation CV | <20% (total protein, chemiluminescence and fluorescence) | N/A | ||
| Dynamic range | 2-3 logs | 3-4 logs | 1 logs | |
| Sensitivity | ng | Low pg | High pg | ng |
| Capillary | 5 cm, 100 µm, 400 nL | |||
| Runtime | <3 hours | <4 hours with immunoassay | ||
| Samples per run | 13 or 25 | |||
| Weight | 23 kg | |||
| Dimensions (closed) | 0.36 m H X 0.3 m W X 0.57 m D | |||
| Dimensions (open) | 0.36 m H x 0.53 m W x 0.57 m D | |||
| Power | US/CAN 120 V AC, 60 hz, 4.2 amps Europe 240 AC, 50 Hz, 2.1 amps Japan 100 AC, 50/60 Hz, 5.0 amps | |||
| Operating temperature | 18-24 °C | |||
| Operating humidity | 20-60% relative, non-condensing | |||
* Inter-assay CV is calculated with normalization to the system control protein
** Percent peak area
Key Features
- Reproducible: Ensures precise control over sample loading, incubations, and washes, eliminating inconsistencies and user-dependent variability in traditional Westerns.
- High throughput: With her 13 and 25 sample capillary cartridges, Jess provides high throughput with minimal hands-on time. Utilize her fluorescent capabilities to multiplex proteins for even greater efficiency.
- Fast: Load your sample, antibodies, and reagents, press start, and in just 3 hours of hands-free runtime.
- Quantitative: Use the lane view to compare band intensity or perform detailed quantitative analysis of protein size and concentration, easily creating standard curves for precise quantification.
Brand
Bio-Techne includes the following brands: ACD, Asuragen, ExosomeDx, Lunaphore, Novus Biologicals, ProteinSimple, R&D Systems and Tocris Bioscience.
Bio-Techne is headquartered in Minneapolis, Minnesota and employs over 3,000 people globally at 34 locations worldwide. As a global developer, manufacturer and supplier of high-quality reagents, analytical instruments and precision diagnostics, Bio-Techne has an extensive catalog of over 500,000 products.