Vazyme – HiScript IV RT SuperMix for qPCR (+gDNA wiper)
HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription.
This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contamination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH2O to start the reaction.
Technical Specifications
| Components | R423-01 100 rxns (20 μl/rxn) |
| RNase-free ddH2O | 2 x 1 ml |
| 5 x gDNA wiper Mix | 300μl |
| 4 x HiScript III qRT SuperMixa | 500μl |
| 4 x No RT Control Mixb | 50μl |
a. It contains HiScript IV Reverse Transcriptase, RNase inhibitor, Random primers/Oligo (dt)20VN primer mix, etc.
b. Except for HiScript IV reverse Transcriptase, the remaining components are consisted with the 4 x HiScript RT qRT SuperMix, which is use to prepare No RT control reaction system.
Key Features
- Superior reverse transcription efficiency: Achieve qPCR detection results with smaller CT values.
- Great gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection.
- Excellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.
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