Bio-techne – Classical Culture Media & Balanced Salt Solutions
Providing ready-to-use products for cell culture, including essential options like DMEM and HBSS, ensures consistent and reliable results and supports optimal cell growth and experimental reproducibility.
Dulbecco’s Modified Eagle’s Medium (DMEM), a modification of Eagle’s BME, is widely used for a range of mammalian cell culture applications. In comparison with BME, it contains a four-fold higher concentration of amino acids, vitamins, and additional components. A variety of DMEM versions are available in high glucose and low glucose formulations. DMEM requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.
Hanks’ Balanced Salt Solution (HBSS) is an isotonic buffer solution containing inorganic salts and a carbohydrate as an energy source. It is used for various cell culture applications including the short -term maintenance of cells in a non-CO2 environment, washing cells before dissociation or counting, and transporting cells or tissues.
Technical Specifications
DMEM
| Model Number | M22450 | M18650 | M22650 | M22250 | M22850 |
|---|---|---|---|---|---|
| Description | high glucose, L-glutamine, sodium pyruvate | high glucose, L-glutamine, sodium pyruvate, no phenol red | high glucose, 25 mM HEPES, L-glutamine, sodium pyruvate | high glucose, L-glutamine, no sodium pyruvate | high glucose, no glutamine, no sodium pyruvate |
| Purification | 0.2 μm filtered | ||||
| Storge | 2 – 8 °C, protected from light | ||||
HBSS
| Model Number | B32350 | B32350 | B32450 |
|---|---|---|---|
| Description | no Ca and Mg salts, no phenol red | no Ca and Mg salts, phenol red | Ca and Mg salts, no phenol red |
| Purification | 0.2 μm filtered | ||
| Storge | 2 – 8 °C | ||
Key Features
DMEM
- High Glucose Variants: Optimized for energy-demanding cellular processes.
- L-Glutamine Options: Provides essential nutrients for protein synthesis and metabolic activity.
- Sodium Pyruvate Choices: Tailored to meet diverse metabolic needs.
- With or Without Phenol Red: Flexible options to minimize interference with sensitive assays.
- HEPES Buffering: Available for enhanced pH stability in fluctuating environmental conditions.
HBSS
- Calcium and Magnesium Variants: Available with or without these salts to support specific experimental needs.
- Phenol Red Options: Choose with or without phenol red based on assay compatibility.
- No Calcium/Magnesium Formulations: Ideal for washing procedures or specific cellular interactions.
Bio-Techne includes the following brands: ACD, Asuragen, ExosomeDx, Lunaphore, Novus Biologicals, ProteinSimple, R&D Systems and Tocris Bioscience. Bio-Techne is headquartered…
