Bio-techne – Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME)
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. The major components of the basement membrane include laminin, collagen IV, entactin/nidogen, and heparan sulfate proteoglycan. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation.
Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane that provides high tensile strength, enhanced levels of entactin/nidogen, elevated protein concentration, and robust clarity and purity. Purified from mouse Engelbreth-Holm-Swarm (EHS) tumor, Cultrex UltiMatrix RGF BME is viscous at 2-8 °C and gels at 37 °C to form a reconstituted basement membrane. Employing an optimized manufacturing process that maximizes purification and solubilization of extracellular matrix protein, Cultrex UltiMatrix RGF BME is a cell scaffolding hydrogel with substantial compositional enhancements and performance benefits for 2D and 3D cell culture applications.
Technical Specifications
| Concentration | 10-12 mg/mL as determined by Lowry assay. |
| Endotoxin | Endotoxin concentration ≤ 7 EU/mL by LAL assay. |
| Source | Murine Engelbreth-Holm-Swarm (EHS) tumor. |
| Storage Buffer | Dulbecco’s Modifi ed Eagle’s Medium without phenol red, containing 50 μg/mL gentamicin sulfate. |
| Stability | Product is stable for three years from date of manufacture when stored at ≤ -70 °C . See lot specifi c Certifi cate of Analysis for expiration date. |
| Storage | Store at ≤ -70 °C. Product may be thawed and dispensed into working aliquots. Avoid freeze-thaw cycles. |
Key features
- Designed for organoid and stem cell culture
- Protein composition and storage modulus optimized for 3D applications
- Qualified for dome formation
- Lot-to-lot performance consistency
- High protein concentration
- Low endotoxin profile
Bio-Techne includes the following brands: ACD, Asuragen, ExosomeDx, Lunaphore, Novus Biologicals, ProteinSimple, R&D Systems and Tocris Bioscience. Bio-Techne is headquartered…
