Vazyme – HiScript III All-in-one RT SuperMix Perfect for qPCR
HiScript III All-in-one RT SuperMix Perfect for qPCR is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper).
Within one step, genomic removal and reverse transcription reactions can be achieved simultaneously. The simpler and faster opereation ensure reduce risk of sample contamination and RNA degradation. This product contains HiScript III Reverse Transcriptase, heat-labile DNase and an optimized buffer system. The heat-labile DNase works quickly and efficiently; and it could be inactivated easily. The RT product is compatible with dye-based and probe-based qPCR, and can perform high-performance gene expression analysis.
Technical Specifications
| Components | R333-01 100 rxns (20 μl/rxn) |
| RNase-free ddH2O | 2 x 1 ml |
| Enzyme Mix* | 100μl |
| 5 x All-in-one qRT SuperMix* | 400μl |
| No RT Control Mix* | 20μl |
*Please keep Enzyme Mix, 5 x All-in-one qRT SuperMix and No RT Control Mix on ice to keep the activity of heat-labile DNase.
Key Features
- Fast and convenient: The gDNA elimination and reverse transcription can be finished in one step. The whole reaction take only 15 min.
- Strong cDNA stability: The heat-labile DNase can be inactivated thoroughly, thus cDNA can be preserved for a longer period of time.
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