Vazyme – Phanta Max Super-Fidelity DNA Polymerase
Phanta Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta Super-Fidelity DNA Polymerase.
Compared with the previous generation, Phanta Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. Phanta Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of Phanta Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity.
Technical Specifications
Components | P505-d1 (100 U) | P505-d2 (500 U) | P505-d3 (1,000 U) |
Phanta Max Super-Fidelity DNA Polymerase | 100μL | 5 x P505-d1 | 10 x P505-d1 |
2 x Phanta Max Buffer | 2 x 1.25 ml | ||
dNTP Mix (10 mM each) | 100μL | ||
10 x DNA Loading buffer | 1.25 ml |
Key Features
- Ultra-high fidelity: ~128X higher than Taq DNA Polymerase
- Longer amplicon lengths: Up to 40 kb simple templates, 10 kb cDNA and 20 kb gDNA
- Robust performance: Superior specificity, sensitivity, and yields
- Broad Compatibility: Suitable for complex samples & GC-rich systems
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