Cytek® – Cytek® Amnis® Imaging Flow Cytometers
The Cytek® Amnis® ImageStream® Mk II imaging flow cytometer combines the high throughput performance of flow cytometry with the imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
The ImageStream Mk II system is a benchtop, multispectral, imaging flow cytometer designed for the rapid acquisition of millions of cells with up to 12 channels of cellular imagery. The instrument collects large numbers of digital images, performs spectral compensation and provides high quality images of every cell in your sample.
Key Features
- Beyond forward and side scatter: Amnis imaging flow cytometers produce familiar ‘size vs. complexity’ scatter plots, but with the power of 20X magnification—or more, report absolute rather than relative cell size by measuring the actual diameter of objects using the brightfield images.
- Exceptional fluorescence sensitivity: Provides extraordinary fluorescence sensitivity across the visible spectrum, outperforming other imaging devices.
- Fast and Easy Software Loaded: Cytek® INSPIRE™ software offers powerful, image-based gating and real time fluorescence compensation
Technical Specifications
| Performance Charactertics | Magnification | ||
| 40x | 60x | 20x | |
| Numeric aperture | 0.75 | 0.9 | 0.5 |
| Pixel size | 0.5 x 0.5 µm | 0.3 x 0.3 µm | 1.0 x 1.0 µm |
| Field of view | 60 x 128 µm | 40 x 170 µm | 120 x 256 µm |
| Imaging rate | 2,000 cells/sec | 1,200 cells/sec | 5,000 cells/sec |
| Sample characteristics | Volume: 20-200 µL Utilization Efficiency: Up to 95% of sample |
| Physical characteristics | 35” W x 26” H x 25” D in (889 mm x 660 mm x 635 mm) 400 lbs. (182 kg) |
| Illumination | Excitation: Standard: 488 nm; Optional: High Power 488, 375 nm, 405 nm, 561 nm, 592 nm, and 642 nm Side scatter: 785 nm standard Brightfield: Multi-channel |
| Operational requirements | 450W, 100-240 VAC, 50/60 Hz (No external air or water necessary) |
Roswell Park Comprehensive Cancer Center’s Partnership with Cytek® Amnis® Imaging Flow Cytometers
Cytek Biosciences is committed to providing powerful solutions that enable scientists to rapidly and efficiently obtain deep biological insights through…
