Thermo Fisher – Solvents and blends for HPLC applications

  • PHILIPPINES

    DKSH Market Expansion Services Philippines, Inc.
    3rd Floor, Science Hub Tower 2, Campus Avenue,
    McKinley Hill Cyberpark Pinagsama,
    Taguig City, 1630 Philippines

    +63 2 8 548 3200

    Email

  • HPLC Brochure

  • HPLC TFS Food Brochure

  • HPLC TFS QR Brochure

Our chromatography solvents are made specifically for HPLC instrumentation. They are prepared by a distillation process to remove volatile impurities that can contaminate the analytical columns and reduce their lifetimes, followed by filtration to achieve maximum purity. These solvents have low UV absorption and low peak interference and are free of particles up to 0.02 micron. Thus, usage of our high purity HPLC solvents extends column lifetime. Our HPLC grade solvents are also designed to significantly reduce detector noise and baseline drifts, thereby enhancing data quality and helping ensure reproducibility.

Available Models

Technical Specifications

DescriptionPackagingSizesCat. No.
AcetoneAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LA929
AcetonitrileAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LA996
Chloroform (with ~50ppm Amylene as a Preservative)Amber Glass Bottles4LC297
Ethyl AcetateAmber Glass Bottles and Safe-Cote™ Bottles4LE196
Ethyl Acetate/Ethanol 3:1 SolutionAmber Glass Bottles4LE151
HexanesAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LH303
n-Hexane, 95%Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LH306
MethanolAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LA454
Methylene ChlorideAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)4LD151
Petroleum EtherAmber Glass Bottles and Safe-Cote™ Bottles4LE120
2-PropanolAmber Glass Bottles and Safe-Cote™ Bottles4LA464
TetrahydrofuranAmber Glass Bottles and Safe-Cote™ Bottles (4 L Only)1 and 4 LT427
TolueneAmber Glass Bottles and Safe-Cote™ Bottles4LT291
WaterAmber Glass Bottles and Safe-Cote™ Bottles4LW7

Fisher Chemical™ Optima™ grade solvents meet high purity requirements and are the choices for HPLC and GC, spectrophotometry, environmental testing and other analytical applications.

Key Features

  • Screened for fluorescent and UV-absorbing contaminants
  • Meet ACS specifications
  • Certificates of analysis and SDS available online and by scanning the QR codes on the label
  • Many tested for pesticides via GC ECD

Technical Specifications

DescriptionSizesCat. No.
Acetic Acid, Glacial (HPLC), Fisher Chemical™500 mL, 1 LA/0406
Acetone (HPLC), Fisher Chemical™1 L, 2.5 LA/0606
Acetonitrile, for HPLC, Fisher Chemical™1 L, 2.5 L, 5 LA/0626
Acetonitrile, HPLC for Gradient Analysis, meets analytical specification of Ph.Eur, Fisher Chemical™1 L, 2.5 L, 5 LA/0627
Acetonitrile, Certified, for HPLC-RMN with Low Propionitrile Level1 L, 2.5 LA/0636
n-Butyl Chloride (HPLC), Fisher Chemical™1 L, 2.5 LC/4756
Chloroform, Stabilized with Amylene, for HPLC, Fisher Chemical™1 L, 2.5 LC/4966
Cyclohexane, for HPLC, Fisher Chemical™1 L, 2.5 LC/8936
1,2-Dichloroethane, for HPLC, Fisher Chemical™1 L, 2.5 LD/1756
Dichloromethane, for HPLC, Stabilised with Amylene, Fisher Chemical™1 L, 2.5 L, 5 LD/1856
Dichloromethane, for HPLC, Unstabilised, Fisher Chemical™1 L, 2.5 LD/1857
Diethyl Ether, for HPLC, Stabilised with Ethanol, Fisher Chemical™1 L, 2.5 LD/2506
DimethylFormamide, for HPLC, Fisher Chemical™1 L, 2.5 LD/3846
Dimethyl Sulfoxide, for HPLC, Fisher Chemical™250 mL, 500 mL, 2.5 LD/4125
1,4-Dioxane, 99.5+%, for HPLC, Unstabilised, Fisher Chemical™1 L, 2.5 LD/4556
Ethanol Absolute, for HPLC, Fisher Chemical™1 L, 2.5 LE/0665
Ethyl Acetate, for HPLC, Fisher Chemical™1 L, 2.5 LE/0906
Heptane, for HPLC, approx. 99% n-Heptane, Fisher Chemical™1 L, 2.5 LH/0106
Heptane, HPLC for fluorescence detection, approx. 99% n-Heptan…1 L, 2.5 LH/0107
Isohexane, for HPLC, contains <5% n-Hexane, Fisher Chemical™1 L, 2.5 L, 5 L, 25 LH/0405
Hexanes, for HPLC, 95% n-Hexane approx., Fisher Chemical™1 L, 2.5 L, 5 LH/0406
Hexanes, HPLC for fluorescence detection, 95% n-Hexane approx., Fisher Chemical™1 L, 2.5 LH/0409
Methanol, for HPLC, Fisher Chemical™1 L, 2.5 L, 5 LM/4056
Methanol, HPLC for Gradient Analysis, Fisher Chemical™1 L, 2.5 L, 5 LM/4058
Methyl-Tert-Butyl Ether, for HPLC, Fisher Chemical™2.5 LM/4496
Pentane, for HPLC, Mixed Isomers, Fisher Chemical™1 L, 2.5 LP/1006
Propan-1-ol, for HPLC, Fisher Chemical™1 L, 2.5 LP/7486
Isopropanol, for HPLC, Fisher Chemical™1 L, 2.5 LP/7507
Tetrahydrofuran, for HPLC, Unstabilised, Fisher Chemical™1 L, 2.5 LT/0706
Toluene, for HPLC, Fisher Chemical™1 L, 2.5 LT/2306
2,2,4-Trimethylpentane (Isooctane), for HPLC, Fisher Chemical™1 L, 2.5 L, 5 LT/3606

HPLC grade solvents are specifically for use in analytical and preparatory liquid chromatography.

Key Features

  • Meet ACS specifications
  • Submicron filtered
  • Supplied in specially cleaned bottles
  • Blanketed with inert gas to maintain integrity
  • Certificate of Analysis available online
  • Scan QR codes on label for CofA and SDS
  • Bulk size options available

Technical Specifications

DescriptionPackagingSizesCat. No.
Acetonitrile with 0.1% FAAmber Glass Bottles4LHB9823-4
Acetonitrile with 0.05% TFAAmber Glass Bottles4LHB9812-4
Acetonitrile with 0.1% TFAAmber Glass Bottles4LHB9813-4
Mobile Phase Buffer, HPLC, pH 10, 10mM Ammonium Bicarbonate, 5% MethanolAmber Glass Bottles4LT006024000
Water with 0.1% FAAmber Glass Bottles4LHB523-4
Water with 0.05% TFAAmber Glass Bottles4LHB512-4
Water with 0.1% TFAAmber Glass Bottles4LHB513-4

HPLC mobile phase blends meet the strict purity requirements of HPLC and provide a consistent formulations of formic acid (FA) or trifluoroacetic acid (TFA) with a low LC/UV background. Pre-blended mobile phases offer convenience and consistency in your HPLC operation and avoid the contamination, quality and safety risks often associated with blending solvents and acids in-house.

Key Features

  • Ready-to-use
  • Submicron filtered
  • LC/UV tested at 210nm and 254nm
  • Lot-to-lot consistency
  • Packaged in specially treated bottles and sealed with FisherLOCK caps

Improving Separation in Reversed Phase HPLC

High performance liquid chromatography

Application Note 

Separation in reversed phase liquid chromatography depends on non-polar analytes being retained by a hydrophobic stationary phase such as a C18 column. If the sample contains ionic (polar) components, they may not be sufficiently retained by the stationary phase for adequate separation. The primary methods of improving retention are via ion pairing or pH modification.

Key Industries

  • Aerospace
  • Agriculture
  • Automotive
  • Biopharmaceutical and Biotechnology
  • Chemical
  • Construction
  • Cosmetics & Personal Care
  • Defense
  • Die & Mould
  • Education & Academics
  • Electronics
  • Energy
  • Environmental
  • Food & Beverage
  • Luxury
  • Machinery & Manufacturing
  • Medical
  • Mining & Minerals
  • Oil & Gas
  • Optical
  • Paper & Wood
  • Pharmaceutical
  • Polymers, Plastics and Rubbers
  • Powders & Pigments / Coating
  • Railway
  • Semiconductor, Solar & Electronics
  • Service
  • Shipbuilding
  • Textile
  • Wholesale & Retail

Brand

Thermo Fisher Scientific

Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services.

test block for Laos

Your download – just a few steps away

Please enter your details here:

test

Contact us