Vazyme – HiScript III RT SuperMix for qPCR (+gDNA Wiper)

HiScript III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. 

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Overview

Features

Specifications

This kit further improves the efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning an exon-exon junction; 5 × HiScript III qRT SuperMix contains all the components required for the reverse transcription reaction. The reaction can be performed quickly by adding template RNA and RNase-free ddH2O, and at the same time, the gDNA wiper function is terminated to ensure the integrity of the cDNA. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.

Technical Specifications 

Components R323-01 100 rxns (20 μl/rxn) 
RNase-free ddH22 x 1 ml 
4 x gDNA wiper Mix 400μl 
5 x HiScript III qRT SuperMixa 400μl 
5 x No RT Control Mixb 40μl 

a. It contains Buffer, dNTP, HiScript III Reverse Transcriptase, RNase inhibitor, Random primers/Oligo (dt)20VN primer Mix 
b. It does not contain HiScript II reverse transcriptase. Other components are the same as 5 x HiScript ii SuperMix for the preparation of No RT Control reaction. 

Key Features

  • Easy operation: Add RNA templates to initiate the reverse transcription reaction. It takes only 18 min to finish gDNA elimination and reverse transcription.
  • Broad template compatibility: It is compatible with various species and degraded RNA.
  • Strong impurity tolerance ability: It has strong tolerance to common reverse transcription impurities such as ethanol, isopropyl alcohol, water-balanced phenol, guanidine isothiocyanate, humic acid, etc.
  • Great cDNA synthesis efficiency: In qPCR analysis, cDNA obtained with Vazyme #R323 has smaller CT values.

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