Vazyme

The automatic nucleic acids extraction instrument is a high-throughput and high-precision nucleic acid extractor used in pair with prepackaged extraction reagents based on silica-coated superparamagnetic beads to extract and purify nucleic acids from the blood, tissue, cells, body fluids, bacteria, viruses, and many other biological samples. It purifies and enriches nucleic acids by utilizing magnetic rods to capture, transfer, and release magnetic beads. With highly automated, fast, and simple workflow, the product is ideal for a wide  range of applications in molecular diagnosis and animal disease detection.

FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit is suitable for extracting genomic DNA from ≤200 μl of fresh or frozen anticoagulated whole blood, <25 mg of animal tissues, <5 × 106 of cultured cells and <3 × 109 cultured bacterial samples. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation step. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, enzyme digestion and virus detection.

The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total  RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology  and does not require β-mercaptoethanol, phenol/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure  gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA  Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The  isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can  be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.

FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. 

The FMR3 instrument consists of control system, power system, electro-optic system, module component, heated-lid component, shell component and software (Version V1.0).

This instrument is intended to use with proper reagents together to carry quantitative/qualitative detection and melt curve analysis for nucleic acid derived from human body based on fluorescent polymerase chain reaction (PCR), such as pathogen and human nucleic acid, etc.

FreeZol Reagent is widely applicable to extracting total RNA from cultured cells, animal tissues, and simple plant tissues. Compared with the conventional Trizol method, this product features a simple procedure that can be performed at room temperature with no need to use chloroform for phase separation. In addition, this product ensures the integrity and purity of the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, NGS, and other molecular biology experiments.

HiScript III All-in-one RT SuperMix Perfect for qPCR is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper). Within one step, genomic removal and reverse transcription reactions can be achieved simultaneously. The simpler and faster opereation ensure reduce risk of sample contamination and RNA degradation. This product contains HiScript III Reverse Transcriptase, heat-labile DNase and an optimized buffer system. The heat-labile DNase works quickly and efficiently; and it could be inactivated easily. The RT product is compatible with dye-based and probe-based qPCR, and can perform high-performance gene expression analysis.

HiScript III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. This kit further improves the efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning an exon-exon junction; 5 × HiScript III qRT SuperMix contains all the components required for the reverse transcription reaction. The reaction can be performed quickly by adding template RNA and RNase-free ddH2O, and at the same time, the gDNA wiper function is terminated to ensure the integrity of the cDNA. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.

HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contamination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH2O to start the reaction.

Phanta Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with the previous generation, Phanta Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. Phanta Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of Phanta Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity.

2 × Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec/kb, greatly saving reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. This product contains loading buffer and tracking dye, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use.

Taq Pro Universal SYBR qPCR Master Mix is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Taq Pro DNA Polymerase, is a new generation of hot-start polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity and high amplification yield. With the combination of Buffer optimized for qPCR and specific enhancer, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains Specific ROX Reference Dye, which is suitable for all qPCR instruments without adjusting the concentration of ROX on different instruments. This kit contains 2 × master mix that can be amplified by adding primers and template.

VAMNE MagUltra Blood Genomic DNA Extraction Kit is intended for fast and easy genomic DNA (gDNA) isolation from 50 μl – 1 ml of fresh or frozen anticoagulated whole blood samples. This kit utilizes superparamagnetic particle paried with unique reagents for the effective removal of various impurities and specific adsorption of gDNA. The workflow does not require organic reagents such as phenol or chloroform, and highly purified gDNA can be obtained reproducibly within 1 h. The extracted gDNA can be directly used for downstream experiments, such as enzyme digestion, PCR, qPCR, library preparation, and Southern blot.